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The glycoprotein fibulin‐3 regulates morphology and motility of olfactory ensheathing cells in vitro
Author(s) -
Vukovic Jana,
Ruitenberg Marc J.,
Roet Kasper,
Franssen Elske,
Arulpragasam Ajanthy,
Sasaki Takako,
Verhaagen Joost,
Harvey Alan R.,
Busfield Samantha J.,
Plant Giles W.
Publication year - 2009
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20771
Subject(s) - fibulin , biology , olfactory ensheathing glia , microbiology and biotechnology , neurite , olfactory epithelium , olfactory mucosa , extracellular matrix , olfactory system , neuroscience , olfactory bulb , central nervous system , in vitro , genetics
Abstract The primary olfactory pathway in adult mammals has retained a remarkable potential for self‐repair. A specialized glial cell within the olfactory nerve, called olfactory ensheathing cell (OEC), and their associated extracellular matrix are thought to play an important role during regenerative events in this system. To gain insight into novel molecules that could mediate the OEC‐supported growth of axons within the olfactory nerve, gene expression profiling experiments were conducted which revealed high expression of the glycoprotein fibulin‐3 in OECs. This observation was confirmed with quantitative PCR. In vivo , the distribution of all members of the fibulin family, fibulin‐3 included, was localized to the lamina propria underneath the olfactory epithelium, in close association within olfactory nerve bundles. To manipulate fibulin‐3 gene expression in cultured OECs, lentiviral vector constructs were designed to either transgenically express or knock‐down fibulin‐3. Experimental data showed that increased levels of fibulin‐3 induced profound morphological changes in cultured OECs, impeded with their migratory abilities and also suppressed OEC‐mediated neurite outgrowth. Knock‐down of fibulin‐3 levels resulted in reduced OEC proliferation. In conclusion, the data provide novel insights into a putative role for fibulin‐3 in the regulation of cell migration and neurite outgrowth within the primary olfactory pathway. © 2008 Wiley‐Liss, Inc.