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GLT‐1 expression and Glu uptake in rat cerebral cortex are increased by phencyclidine
Author(s) -
Fattorini Giorgia,
Melone Marcello,
Bragina Luca,
Candiracci Chiara,
Cozzi Andrea,
Pellegrini Giampietro Domenico E.,
TorresRamos Monica,
PérezSamartín Alberto,
Matute Carlos,
Conti Fiorenzo
Publication year - 2008
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20700
Subject(s) - phencyclidine , microdialysis , astrocyte , glutamate receptor , biology , cerebral cortex , extracellular , transporter , nmda receptor , blot , hippocampus , pharmacology , cortex (anatomy) , neuroscience , biochemistry , medicine , central nervous system , receptor , gene
Using western blottings, microdialysis, and functional assays we tested the hypothesis that phencyclidine (PCP) modifies the expression and function of glutamate (Glu) transporters in the rat frontal cortex. Western blotting studies revealed that administration of PCP (10 mg/kg/day; 7 days) increased significantly the expression of the astrocytic Glu transporter GLT‐1/EAAT2. Functional studies showed that PCP increased significantly Na + ‐dependent Glu uptake in slices and in neuron/astrocyte co‐cultures, and microdialysis studies evidenced that PCP treatment reduced basal Glu output. In our experimental conditions, PCP did not induce toxicity. These studies show that PCP increases the expression of GLT‐1 in the cerebral cortex, thereby increasing Glu uptake and reducing extracellular [Glu]. © 2008 Wiley‐Liss, Inc.