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The ABCA1 cholesterol transporter associates with one of two distinct dystrophin‐based scaffolds in Schwann cells
Author(s) -
Albrecht Douglas E.,
Sherman Diane L.,
Brophy Peter J.,
Froehner Stanley C.
Publication year - 2008
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20636
Subject(s) - biology , dystrophin , utrophin , microbiology and biotechnology , cytoskeleton , schwann cell , gene isoform , scaffold protein , cell , biochemistry , duchenne muscular dystrophy , signal transduction , genetics , gene
Cytoskeletal scaffolding complexes help organize specialized membrane domains with unique functions on the surface of cells. In this study, we define the scaffolding potential of the Schwann cell dystrophin glycoprotein complex (DGC) by establishing the presence of four syntrophin isoforms, (α1, β1, β2, and γ2), and one dystrobrevin isoform, (α‐dystrobrevin‐1), in the abaxonal membrane. Furthermore, we demonstrate the existence of two separate DGCs in Schwann cells that divide the abaxonal membrane into spatially distinct domains, the DRP2/periaxin rich plaques and the Cajal bands that contain Dp116, utrophin, α‐dystrobrevin‐1 and four syntrophin isoforms. Finally, we show that the two different DGCs can scaffold unique accessory molecules in distinct areas of the Schwann cell membrane. Specifically, the cholesterol transporter ABCA1, associates with the Dp116/syntrophin complex in Cajal bands and is excluded from the DRP2/periaxin rich plaques. © 2008 Wiley‐Liss, Inc.