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NG2‐positive cells generate A2B5‐positive oligodendrocyte precursor cells
Author(s) -
Baracskay Karen L.,
Kidd Grahame J.,
Miller Robert H.,
Trapp Bruce D.
Publication year - 2007
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20519
Subject(s) - biology , progenitor cell , oligodendrocyte , microbiology and biotechnology , platelet derived growth factor , remyelination , platelet derived growth factor receptor , progenitor , stem cell , growth factor , immunology , neuroscience , receptor , genetics , myelin , central nervous system
Cellular specification of the oligodendrocyte lineage occurs through a series of stages identified by expression of distinct biochemical characteristics. The best characterized oligodendrocyte progenitor cell (OPC) in vitro is the bipotential O2‐A progenitor, identified by labeling with monoclonal antibody A2B5, which proliferates predominantly in response to platelet derived growth factor (PDGF). The cellular ancestors of O2‐A progenitor cells are currently unclear. In vivo OPCs can be identified by expression of the cell surface markers NG2 (a sulfated proteoglycan) and platelet derived growth factor receptor αR). Substantial evidence supports the generation of oligodendrocytes from NG2 + , PDGFαR + cells both in vivo and in vitro . The developmental relationship between NG2 + cells and A2B5 − positive cells is unknown and it is unclear whether they represent identical, partially overlapping or nonoverlapping populations of cells. Here we show that in cultures of developing brain NG2 + and A2B5 + cells arise from overlapping cell populations. NG2 + cells appear prior to the expression of A2B5 + cells and generate A2B5 + cells. We propose that during development NG2 + /A2B5 − cells (pre‐OPCs) represent the direct ancestor to A2B5 + O2A progenitor cells (OPCs). © 2007 Wiley‐Liss, Inc.