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Alterations in the oligodendrocyte lineage, myelin, and white matter in adult mice lacking the chemokine receptor CXCR2
Author(s) -
PadovaniClaudio Dolly A.,
Liu Liping,
Ransohoff Richard M.,
Miller Robert H.
Publication year - 2006
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20383
Subject(s) - oligodendrocyte , biology , white matter , myelin , cxc chemokine receptors , myelin basic protein , spinal cord , central nervous system , glial fibrillary acidic protein , axon , neuroscience , proteolipid protein 1 , chemokine receptor , endocrinology , chemokine , immunology , immunohistochemistry , medicine , inflammation , radiology , magnetic resonance imaging
Abstract Oligodendrocyte precursor cell (OPC) proliferation and migration are critical for the development of myelin in the central nervous system (CNS). Previous studies showed that localized expression of the chemokine CXCL1 signals through the receptor CXCR2 to inhibit the migration and enhance the proliferation of spinal cord OPCs during development. Here, we report structural and functional alterations in the adult CNS of Cxcr2 −/− mice. In Cxcr2 −/− adult mice, we observed regional alterations in the density of oligodendrocyte lineage cells in Cxcr2 −/− adult mice, with decreases in the cortex and anterior commissure but increases in the corpus callosum and spinal cord. An increase in the density and arborization of spinal cord NG2 positive cells was also observed in Cxcr2 −/− adult mice. Compared with wild‐type (WT) littermates, Cxcr2 −/− mice exhibited a significant decrease in spinal cord white matter area, reduced thickness of myelin sheaths, and a slowing in the rate of central conduction of spinally elicited evoked potentials without significant changes in axonal caliber or number. Biochemical analyses showed decreased levels of myelin basic protein (MBP), proteolipid protein (PLP), and glial fibrillary acidic protein (GFAP). In vitro studies showed reduced numbers of differentiated oligodendrocytes in Cxcr2 −/− spinal cord cultures. Together, these findings indicate that the chemokine receptor CXCR2 is important for the development and maintenance of the oligodendrocyte lineage, myelination, and white matter in the vertebrate CNS. © 2006 Wiley‐Liss, Inc.