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Purkinje cell dendrites grow in alignment with Bergmann glia
Author(s) -
Lordkipanidze Tamar,
Dunaevsky Anna
Publication year - 2005
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20200
Subject(s) - biology , purkinje cell , glial fibrillary acidic protein , dendrite (mathematics) , neuroscience , cerebellum , microbiology and biotechnology , green fluorescent protein , immunohistochemistry , biochemistry , immunology , gene , geometry , mathematics
The pattern of growth of Purkinje cell dendrites has been analyzed and related to their interactions with Bergmann glial radial processes. In cerebellar slice cultures from mice expressing green fluorescent protein (GFP) under the glial fibrillary acidic protein (GFAP) promoter, Purkinje cells were transfected and imaged with two‐photon microscopy over 2 days. We report that while the Purkinje cell dendritic tree grows, individual dendrites increase or decrease in length. Importantly, we demonstrate that vertical growth of Purkinje cell dendrites occurs primarily in alignment with radial glial processes. These findings suggest that radial glial processes provide a structural substrate for the directional growth of Purkinje cell dendrites, thus influencing the shape of the dendritic tree. © 2005 Wiley‐Liss, Inc.