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Mechanical strain injury increases intracellular sodium and reverses Na + /Ca 2+ exchange in cortical astrocytes
Author(s) -
Floyd Candace L.,
Gorin Fredric A.,
Lyeth Bruce G.
Publication year - 2005
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20183
Subject(s) - glutamate receptor , excitotoxicity , astrocyte , traumatic brain injury , intracellular , sodium calcium exchanger , fura 2 , calcium in biology , calcium , sodium , biology , pharmacology , chemistry , medicine , microbiology and biotechnology , neuroscience , biochemistry , central nervous system , enzyme , receptor , psychiatry , cytosol , organic chemistry
Traditionally, astrocytes have been considered less susceptible to injury than neurons. Yet, we have recently shown that astrocyte death precedes neuronal death in a rat model of traumatic brain injury (TBI) (Zhao et al.: Glia 44:140–152, 2003). A main mechanism hypothesized to contribute to cellular injury and death after TBI is elevated intracellular calcium ([Ca 2+ ] i ). Since calcium regulation is also influenced by regulation of intracellular sodium ([Na + ] i ), we used an in vitro model of strain‐induced traumatic injury and live‐cell fluorescent digital imaging to investigate alterations in [Na + ] i in cortical astrocytes after injury. Changes in [Na + ] i , or [Ca 2+ ] i were monitored after mechanical injury or L ‐glutamate exposure by ratiometric imaging of sodium‐binding benzofuran isophthalate (SBFI‐AM), or Fura‐2‐AM, respectively. Mechanical strain injury or exogenous glutamate application produced increases in [Na + ] i that were dependent on the severity of injury or concentration. Injury‐induced increases in [Na + ] i were significantly reduced, but not completely eliminated, by inhibition of glutamate uptake by DL ‐threo‐β‐benzyloxyaspartate (TBOA). Blockade of sodium‐dependent calcium influx through the sodium‐calcium exchanger with 2‐[2‐[4‐(4‐Nitrobenzyloxy)phenyl]ethyl]isothiourea mesylate (KB‐R7943) reduced [Ca 2+ ] i after injury. KB‐R7943 also reduced astrocyte death after injury. These findings suggest that in astrocytes subjected to mechanical injury or glutamate excitotoxicity, increases in intracellular Na + may be a critical component in the injury cascade and a therapeutic target for reduction of lasting deficits after traumatic brain injury. © 2005 Wiley‐Liss, Inc.