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Presence of neuroglobin in cultured astrocytes
Author(s) -
Chen Xiao Qian,
Qin Lu Ye,
Zhang Chen Gang,
Yang Li Tao,
Gao Zhen,
Liu Shuang,
Lau Lok Ting,
Fung YinWan Wendy,
Greenberg David A.,
Yu Albert CheungHoi
Publication year - 2005
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20147
Subject(s) - neuroglobin , biology , immunostaining , homeostasis , astrocyte , microglia , globin , microbiology and biotechnology , neuroglia , apoptosis , neuroscience , immunology , central nervous system , gene , immunohistochemistry , biochemistry , inflammation
Neuroglobin (Ngb), a recently discovered intracellular respiratory globin in neurons, may play a crucial role in oxygen homeostasis in the brain. We report preliminary findings indicating the presence of functional neuroglobin in primary cultures of cerebral cortical astrocytes. Reverse transcription real‐time polymerase chain reaction (RRT–PCR) and immunostaining confirmed such presence in cultured astrocytes isolated from newborn mouse brain. Ngb antisense treatment increased apoptosis in ischemic astrocytes. The discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain. © 2005 Wiley‐Liss, Inc.