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Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats
Author(s) -
Blixt Wojciechowski Anita,
Englund Ulrica,
Lundberg Cecilia,
Warfvinge Karin
Publication year - 2004
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.20033
Subject(s) - retina , transplantation , biology , inner plexiform layer , microbiology and biotechnology , outer plexiform layer , glial fibrillary acidic protein , neural cell adhesion molecule , vimentin , neuroglia , cell adhesion molecule , neuroscience , pathology , immunology , cell , cell adhesion , central nervous system , immunohistochemistry , medicine , biochemistry
Abstract As previously reported, the brain‐derived precursor cell line RN33B has a great capacity to migrate when transplanted to adult brain or retina. This cell line is immortalized with the SV40 large T‐antigen and carries the reporter gene LacZ and the green fluorescent protein GFP. In the present study, the precursor cells were transplanted to the subretinal space of adult rats and investigated early after grafting. The purpose was to demonstrate the migration of the grafted cells from the subretinal space into the retina and the glial cell response of the host retina. Detachment caused by the transplantation method was persistent up to 4 days after transplantation, and then reattachment occurred. The grafted cells were shown to migrate in between the photoreceptor cells before entering into the plexiform layers. Molecules involved in migration of immature neuronal cells as the polysialylated neural cell adhesion molecule (PSA‐NCAM) and the collapsing response‐mediated protein 4 (TUC‐4) was found in the plexiform layers of the host retina, but not in the grafted cells. The expression of the intermediate filaments GFAP, vimentin, and nestin was intensely upregulated immediately after transplantation. A less pronounced upregulation was observed on sham‐operated animals. In summary, the RN33B cell line migrated promptly posttransplantation and settled preferably into the plexiform layers of the retina, the same layers where the migration cues PSA‐NCAM and TUC‐4 were established. In addition, both the transplantation method per se and the implanted cells caused an intense glial cell response by the host retina. © 2004 Wiley‐Liss, Inc.