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Developmental apoptosis in the spinal cord white matter in neonatal rats
Author(s) -
De Louw A.J.A.,
Van De Berg W.D.J.,
De Vente J.,
Blanco C.E.,
Gavilanes A.W.D.,
Steinbusch H.P.J.,
Steinbusch H.W.M.,
Troost J.,
Vles J.S.H.
Publication year - 2002
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.1130
Subject(s) - spinal cord , white matter , immunostaining , biology , apoptosis , microglia , tunel assay , pathology , anatomy , cord , neuroscience , immunohistochemistry , medicine , immunology , magnetic resonance imaging , inflammation , surgery , biochemistry , radiology
We investigated developmental apoptosis in the white matter of the cervical spinal cord at postnatal days 2, 5, and 8. Apoptotic cells were labeled using TUNEL and caspase‐3 immunostaining. Apoptotic cells were diffusely distributed throughout the white matter of the spinal cord. The total amount of apoptotic cells in the cervical spinal cord white matter was related to postnatal age, with the lowest at P2 (mean 7.9, SD 5.6) and the highest at P8 (mean 109, SD 21.4). Using double immunostaining for ED‐1 and O4, apoptotic cells were identified as microglia and oligodendrocytes. GLIA 37:89–91, 2002. © 2002 Wiley‐Liss, Inc.