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Astrocyte and endothelial cell expression of ADAM 17 (TACE) in adult human CNS
Author(s) -
Goddard Diane R.,
Bunning Rowena A.D.,
Woodroofe M. Nicola
Publication year - 2001
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.1060
Subject(s) - biology , astrocyte , receptor , tumor necrosis factor alpha , von willebrand factor , cytokine , endothelial stem cell , immunohistochemistry , pathology , microbiology and biotechnology , immunology , endocrinology , central nervous system , medicine , platelet , biochemistry , in vitro
ADAM 17, also known as TACE, is an important sheddase for a number of proteins, including tumor necrosis factor‐α (TNF‐α), transforming growth factor‐α (TGF‐α), L‐selectin, p75, and p55 TNF receptors, and interleukin‐1 receptor II (IL‐1RII). The presence of ADAM 17 mRNA in adult mouse and rat CNS was recently reported (Karkkainen et al. Mol Cell Neurosci 15:547–560, 2000). However, the cellular origin of ADAM 17 remains unknown. In this study, we have used an anti‐ADAM 17 antibody in an immunohistochemical study of its distribution in human adult CNS tissue. Cells with astrocytic and endothelial morphology were ADAM 17‐positive. This finding was further confirmed using double immunofluorescence with antibodies against GFAP and von Willebrand factor, which label astrocytes and endothelial cells, respectively. This study demonstrates that ADAM 17 is expressed by astrocytes and endothelial cells in normal brain tissue and may have a role in normal brain function. GLIA 34:267–271, 2001. © 2001 Wiley‐Liss, Inc.