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Increased expression of two phospholipase D isoforms during experimentally induced hippocampal mossy fiber outgrowth
Author(s) -
Zhang Yan,
Huang Ping,
Du Guangwei,
Kanaho Yasunori,
Frohman Michael A.,
Tsirka Stella E.
Publication year - 2004
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.10322
Subject(s) - pld2 , neurite , biology , gene isoform , microbiology and biotechnology , hippocampal formation , phospholipase d , neuroscience , gene knockdown , mossy fiber (hippocampus) , in vivo , in vitro , signal transduction , cell culture , biochemistry , phosphatidic acid , phospholipid , dentate gyrus , genetics , membrane , gene
Mammalian phospholipase D (PLD), a multifunctional signaling enzyme, has been reported to facilitate neurite outgrowth in cultured neurons. However, two mammalian isoforms have been found, PLD1 and PLD2, and it has not been determined which isoform is involved, or whether this in vitro phenomenon is relevant to neurite extension in vivo. Using confocal microscopy, we demonstrate that the PLDs are expressed by different cell types in the mouse brain: PLD1 by neurons, and PLD2 by astrocytes. Moreover, using a model of experimentally induced hippocampal mossy fiber sprouting, both isoforms were observed to increase dramatically in expression level along tracts of mossy fiber spouting, supporting the proposal that PLD plays a role in this process. Given that the two isoforms undertake unique molecular functions in cultured cells, our findings suggest that in vivo PLD1 and PLD2 may modulate neuronal plasticity via different pathways and cell types. © 2004 Wiley‐Liss, Inc.