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Antioxidants are necessary for myelination of dorsal root ganglion neurons, in vitro
Author(s) -
Podratz Jewel L.,
Rodriguez Esther H.,
Windebank Anthony J.
Publication year - 2004
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.10302
Subject(s) - ascorbic acid , in vitro , dorsal root ganglion , biology , retinyl palmitate , neuroscience , microbiology and biotechnology , vitamin , medicine , endocrinology , biochemistry , dorsum , anatomy , retinol , food science
We have demonstrated that myelination of dorsal root ganglion (DRG) axons occurs in a fully defined, serum‐free medium (B27). This implies that there may be components in B27 medium that support myelination. To determine which of the components in B27 were essential for myelination, we systematically removed components from B27 until myelination was lost. We added these components to a fully defined minimal medium (N2) that supports neuron survival but not myelination. When antioxidants were removed from B27, myelination was lost. However, the individual antioxidants did not induce myelination when added to N2 medium. Addition of ascorbic acid along with the B27 antioxidants was sufficient to induce myelination in N2 medium, which was enhanced by retinyl acetate. Removal of vitamin E from B27 caused a partial loss of myelination, and addition of vitamin E to N2 medium containing ascorbic acid induced partial myelination. Addition of serum to the B27 myelinating medium inhibited myelination completely. These results indicate that antioxidants are important for myelination, in vitro. Vitamin E may play an important role. Use of a serum‐free medium may be beneficial for in vitro myelination studies because serum has unknown inhibitory effects. © 2003 Wiley‐Liss, Inc.