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Manipulation of olfactory ensheathing cell signaling mechanisms
Author(s) -
Hayat Shaista,
Thomas Anu,
Afshar Farid,
Sonigra Rakesh,
Wigley Caroline B.
Publication year - 2003
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.10299
Subject(s) - neurite , pertussis toxin , olfactory ensheathing glia , biology , microbiology and biotechnology , depolarization , retinal ganglion cell , neuroscience , cholera toxin , g protein , olfactory bulb , signal transduction , central nervous system , retina , biophysics , endocrinology , biochemistry , in vitro
Pretreatment of olfactory ensheathing cells (OECs) with Pertussis toxin increased the number of subsequently cocultured adult retinal ganglion cells (RGCs) regrowing neurites without affecting neuronal survival. Pertussis toxin (PTx) inactivated an OEC G i/o protein as pretreating OECs with the PTx B‐oligomer subunit had no effect on RGC neurite regrowth. However, the B‐oligomer was responsible for decreasing the marked orientation of neurite regrowth on the OEC substrate. Simultaneous incubation of OECs with PTx and a depolarizing concentration of KCl abolished the increase in neurite regrowth from cocultured RGCs, but exposure to a depolarizing KCl concentration after OECs had been PTx‐treated had no effect. Our evidence supports the hypothesis that G‐protein‐regulated calcium signaling plays a significant role in OEC support for CNS axonal regeneration. © 2003 Wiley‐Liss, Inc.