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Characterization of A2B5 + glial precursor cells from cryopreserved human fetal brain progenitor cells
Author(s) -
Dietrich Joerg,
Noble Mark,
MayerProschel Margot
Publication year - 2002
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.10116
Subject(s) - biology , progenitor cell , neural stem cell , cryopreservation , population , microbiology and biotechnology , basic fibroblast growth factor , neurosphere , precursor cell , antigen , immunology , neuroscience , stem cell , cell , growth factor , adult stem cell , biochemistry , in vitro , endothelial stem cell , embryo , receptor , demography , sociology
The identification and characterization of human neural precursor cells are critical in extending our understanding of central nervous system development from model animal systems to our own species. Moreover, availability of well‐characterized populations of human cells is of potential value in endeavors ranging from cell transplantation to drug screening. We have isolated a population of continuously dividing glial‐restricted precursor cells from commercially available cryopreserved 18–20 weeks old fetal brain neural progenitor cells. These human glial‐restricted precursor cells are A2B5 + and do not express polysialylated E‐NCAM (PSA‐NCAM). They can be grown as purified populations in serum‐free medium supplemented with basic fibroblast growth factor (bFGF) and can be induced to generate cells with the antigenic characteristics of oligodendrocytes and distinct astrocytic populations. GLIA 40:65–77, 2002. © 2002 Wiley‐Liss, Inc.