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Remyelination of the spinal cord following intravenous delivery of bone marrow cells
Author(s) -
Akiyama Yukinori,
Radtke Christine,
Honmou Osamu,
Kocsis Jeffery D.
Publication year - 2002
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/glia.10102
Subject(s) - remyelination , bone marrow , spinal cord , myelin , biology , oligodendrocyte , schwann cell , pathology , progenitor cell , anatomy , stem cell , medicine , central nervous system , microbiology and biotechnology , immunology , neuroscience
Bone marrow contains a population of pluripotent cells that can differentiate into a variety of cell lineages, including neural cells. When injected directly into the demyelinated spinal cord they can elicit remyelination. Recent work has shown that following systemic delivery of bone marrow cells functional improvement occurs in contusive spinal cord injury and stroke models in rat. We report here that secondary to intravenous introduction of an acutely isolated bone marrow cell fraction (mononuclear fraction) from adult rat femoral bones separated on a density gradient, ultrastructurally defined remyelination occurs throughout a focal demyelinated spinal cord lesion. The anatomical pattern of remyelination was characteristic of both oligodendrocyte and Schwann cell myelination; conduction velocity improved in the remyelinated axons. When the injected bone marrow cells were transfected to express LacZ, β‐galactosidase reaction product was observed in some myelin‐forming cells in the spinal cord. Intravenous injection of other myelin‐forming cells (Schwann cells and olfactory ensheathing cells) or the residual cell fraction of the gradient did not result in remyelination, suggesting that remyelination was specific to the delivery of the mononuclear fraction. While the precise mechanism of the repair, myelination by the bone marrow cells or facilitation of an endogenous repair process, cannot be fully determined, the results demonstrate an unprecedented level of myelin repair by systemic delivery of the mononuclear cells. GLIA 39:229–236, 2002. © 2002 Wiley‐Liss, Inc.