Estimating and Testing Pleiotropy of Single Genetic Variant for Two Quantitative Traits

ABSTRACT Along with the accumulated data of genetic variants and biomedical phenotypes in the genome era, statistical identification of pleiotropy is of growing interest for dissecting and understanding genetic correlations between complex traits. We proposed a novel method for estimating and testing pleiotropic effect of a genetic variant on two quantitative traits. Based on a covariance decomposition and estimation, our method quantifies pleiotropy as the portion of between‐trait correlation explained by the same genetic variant. Unlike most multiple‐trait methods that assess potential pleiotropy (i.e., whether a variant contributes to at least one trait), our method formulates a statistic that tests exact pleiotropy (i.e., whether a variant contributes to both of two traits). We developed two approaches (a regression approach and a bootstrapping approach) for such test and investigated their statistical properties, in comparison with other potential pleiotropy test methods. Our simulation shows that the regression approach produces correct P ‐values under both the complete null (i.e., a variant has no effect on both two traits) and the incomplete null (i.e., a variant has effect on only one of two traits), but requires large sample sizes to achieve a good power, when the bootstrapping approach has a better power and produces conservative P ‐values under the complete null. We demonstrate our method for detecting exact pleiotropy using a real GWAS dataset. Our method provides an easy‐to‐implement tool for measuring, testing, and understanding the pleiotropic effect of a single variant on the correlation architecture of two complex traits.