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Apolipoprotein B‐gene DNA polymorphisms (X ba l and Eco RI), serum lipids, and apolipoproteins in healthy Chinese
Author(s) -
Saha N.,
Tay J. S. H.,
Humphries S. E.,
Vogler G. P.
Publication year - 1992
Publication title -
genetic epidemiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.301
H-Index - 98
eISSN - 1098-2272
pISSN - 0741-0395
DOI - 10.1002/gepi.1370090103
Subject(s) - apolipoprotein b , ecori , medicine , restriction fragment length polymorphism , biology , genotype , blood lipids , endocrinology , allele , body mass index , haplotype , lipoprotein , cholesterol , polymorphism (computer science) , genetics , gene , restriction enzyme
The frequency of restriction fragment length polymorphisms (RFLPs) of the apolipoprotein B (apo B) gene, detected by Xba I and Eco RI, and their influence on serum lipids and apolipoproteins were studied in healthy Chinese of both sexes in Singapore. A total of 221 subjects (150 males, 71 females) were investigated for the Xba I and 159 subjects for the Eco RI polymorphisms, while serum lipids and apolipoprotein levels were available for 196 subjects. The frequency of the X2 allele was found to be significantly lower in the Chinese than that reported in Caucasians from the United Kingdom (0.09 vs. 0.51, P < 0.001). The haplotype frequencies were also significantly different between the Chinese and Caucasians with a higher frequency of XlRl in the former compared to the latter (0.85 vs. 0.34, P <0.0001). The distribution of RFLP genotypes at both of the restriction sites was at Hardy‐Weinberg equilibrium in all groups. The influence of the apo B RFLPs on serum lipids and apolipoprotein levels (apo AI, AII, and B) was studied by both residual and multiple regression analyses considering age, sex, body mass index (BMI), and genotypes as independent variables in all possible combinations. No association was observed between the apo B genotypes and serum lipids or apolipoprotein levels except for high density lipoprotein cholesterol (HDLC), apo AI and AII, with the X2 being associated with significantly lower levels of HDLC as well as apo AI and AII, the effect being stronger in males. These data raise the possibility that the mechanism of reported association between apo B polymorphism and coronary artery disease may be through effects on HDLC. © 1992 Wiley‐Liss, Inc.