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HLA class II typing using oligonucleotide probes
Author(s) -
Robinson David M.,
Holbeck Susan,
Seyfried Christine,
Byers Patricia,
Palmer Jerry,
Nepom Gerald T.
Publication year - 1989
Publication title -
genetic epidemiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.301
H-Index - 98
eISSN - 1098-2272
pISSN - 0741-0395
DOI - 10.1002/gepi.1370060107
Subject(s) - typing , multiplex , genotyping , oligonucleotide , locus (genetics) , biology , oligomer restriction , genetics , complementary dna , human leukocyte antigen , allele , multiplex ligation dependent probe amplification , microbiology and biotechnology , computational biology , dna , genotype , gene , exon , antigen
A DQβ locus‐specific oligonucleotide probe is used to identify DQβ alleles present in six insulin‐dependent diabetes mellitus (IDDM) multiplex families. Results of genotyping using this technique agree with those from cDNA probes and are often clearer. Allele‐specific oligonucleotide probes for the DRβ locus are used to identify subtypes (Dw4 and Dw14) of the DR4 specificity in five IDDM multiplex families. This distinction cannot be made with available cDNA probes.