Premium
Neuron‐specific enolase‐cre mouse line with cre activity in specific neuronal populations
Author(s) -
Kwon ChangHyuk,
Zhou Jing,
Li Yanjiao,
Kim Ki Woo,
Hensley Lori L.,
Baker Suzanne J.,
Parada Luis F.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20197
Subject(s) - enolase , biology , transgene , genetically modified mouse , premovement neuronal activity , neuron , microbiology and biotechnology , hippocampus , gene , neuroscience , immunohistochemistry , genetics , immunology
To establish genetic tools for conditional gene deletion in mouse neurons, we generated two independent neuron‐specific enolase ( Nse )‐ cre transgenic lines. The transgenic line termed Nse‐cre CK1 showed cre activity in most neuronal regions in the nervous system, while the Nse‐cre CK2 line exhibited a unique cre activity that has not been reported in other cre transgenic lines. Nse‐cre CK2 cre activity was detectable from embryogenesis and mostly restricted to neuronal regions. In postnatal brain, the Nse‐cre CK2 line exhibited cre activity limited to differentiated neurons in the cerebral cortex and hippocampus. Cre activity was assayed in several internal organs and sporadic activity was limited to the kidney and testis. We conclude that these cre lines will be useful for studying loss of gene function in specific neuronal populations. genesis 44:130–135, 2006. Published 2006 Wiley‐Liss, Inc.