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Optimized β‐galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos
Author(s) -
Kishigami Satoshi,
Komatsu Yoshihiro,
Takeda Haruko,
NomuraKitabayashi Aya,
Yamauchi Yasutaka,
Abe Kuniya,
Yamamura Kenichi,
Mishina Yuji
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20186
Subject(s) - staining , endogeny , microbiology and biotechnology , embryo , in situ hybridization , biology , gene , beta galactosidase , gene expression , biochemistry , genetics
β‐Galactosidase (β‐gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6‐chloro‐3‐indoxyl‐beta‐D‐galactopyranoside (S‐gal) is more sensitive for β‐gal activity than 5‐bromo‐4‐chloro‐3‐indolyl‐beta‐D‐galactoside (X‐gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β‐gal staining with S‐gal and in situ hybridization using DIG‐labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S‐gal. genesis 44:57–65, 2006. © 2006 Wiley‐Liss, Inc.