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Synapsin I Cre transgene expression in male mice produces germline recombination in progeny
Author(s) -
Rempe D.,
Vangeison G.,
Hamilton J.,
Li Y.,
Jepson M.,
Federoff H.J.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20183
Subject(s) - transgene , biology , germline , synapsin i , cre recombinase , genetically modified mouse , allele , genetics , microbiology and biotechnology , gene , cre lox recombination , vesicle , membrane , synaptic vesicle
Abstract The cre/ LoxP system can produce conditional loss of gene function in specific cell types such as neurons. A transgenic mouse line, utilized by multiple studies, used the Synapsin I promoter to drive expression of cre (SynCre) to achieve neuronal‐specific cre expression. Herein we describe that cre expression can also be observed in SynCre mice within the testes after being bred into a floxed transgenic mouse line. Cre transcript was expressed in testes resulting in recombination of the floxed substrate in testes. In the majority of cases, progeny of male SynCre mice inherited a germline recombined floxed allele, while this was never observed in progeny from female mice carrying the SynCre allele. This observation should alert investigators to a potential confound using these mice and enables male germ cell “deletor” strategies. genesis 44:44–49, 2006. © 2006 Wiley‐Liss, Inc.