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Generation and characterization of the PKCγ‐Cre mouse line
Author(s) -
Ding YuQiang,
Xiang ChuanXi,
Chen ZhouFeng
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20151
Subject(s) - cre recombinase , biology , microbiology and biotechnology , gene isoform , genetically modified mouse , central nervous system , spinal cord , gene knockin , knockout mouse , endogeny , protein kinase c , transgene , neuroscience , kinase , gene , biochemistry
PKCgγ is a protein kinase C (PKC) isoform that is expressed in the central nervous system (CNS). We generated a PKCgγ‐Cre mouse line and characterized the expression and activity of the Cre protein. Our studies show that Cre expression largely recapitulates the endogenous expression of PKCgγ. Several major sites of Cre expression are the cerebral cortex, hippocampus, thalamus, amygdala, and spinal cord. Examination of PKCgγ‐Cre/ROSA26 mice reveals a similar X‐gal staining pattern in the CNS, indicating that Cre recombinase is capable of removing LoxP sites in vivo. These data indicate that the PKCgγ‐Cre mouse line could be a useful reagent for generating Cre‐mediated tissue‐specific knockouts in the CNS. genesis 43:28–33, 2005. © 2005 Wiley‐Liss, Inc.