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GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless
Author(s) -
Dornan Anthony J.,
Gailey Donald A.,
Goodwin Stephen F.
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20143
Subject(s) - enhancer , biology , enhancer trap , promoter , locus (genetics) , genetics , reporter gene , mutant , gene , phenotype , allele , microbiology and biotechnology , gene expression
The fru 4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry + ] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru 4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w + ] , recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru ‐specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3‐ and P4‐derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru ‐expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities. genesis 42:236–246, 2005. © 2005 Wiley‐Liss, Inc.