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Coxsackievirus and adenovirus receptor is essential for cardiomyocyte development
Author(s) -
Asher Damon R.,
Cerny Anna M.,
Weiler Sarah R.,
Horner James W.,
Keeler Marilyn L.,
Neptune Mychell A.,
Jones Stephen N.,
Bronson Roderick T.,
DePinho Ronald A.,
Finberg Robert W.
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20127
Subject(s) - coxsackievirus , biology , apoptosis , myocarditis , receptor , myocyte , microbiology and biotechnology , transmembrane protein , embryonic stem cell , programmed cell death , immunohistochemistry , pathology , cancer research , immunology , medicine , virus , genetics , enterovirus , gene
The coxsackievirus and adenovirus receptor (CAR) is a transmembrane protein that is known to be a site of viral attachment and entry, but its physiologic functions are undefined. CAR expression is maximal in neonates and wanes rapidly after birth in organs such as heart, muscle, and brain, suggesting that CAR plays a role in the development of these tissues. Here, we show that CAR deficiency resulted in an embryonic lethal condition associated with cardiac defects. Specifically, commencing ∼10.5 days postconception (dpc), CAR −/− cardiomyocytes exhibited regional apoptosis evidenced by both histopathologic features of cell death and positive staining for the apoptotic marker cleaved caspase 3. CAR −/− fetuses invariably suffered from degeneration of the myocardial wall and thoracic hemorrhaging, leading to death by 11.5 dpc. These findings are consistent with the view that CAR provides positive survival signals to cardiomyocytes that are essential for normal heart development. genesis 42:77–85, 2005. © 2005 Wiley‐Liss, Inc.