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Targeting gene expression in the mouse somite: Adenovirus‐mediated gene delivery and whole embryo culture
Author(s) -
Chen Alice E.,
Fan ChenMing
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20126
Subject(s) - smoothened , biology , transgenesis , embryonic stem cell , sonic hedgehog , somite , microbiology and biotechnology , embryo , gene , gene delivery , gene expression , genetic enhancement , genetics , hedgehog signaling pathway , embryogenesis , reproductive technology
We report here a novel approach to direct gene expression in the mouse somite based on the combined application of adenovirus‐mediated gene delivery and whole embryo ex vivo culture. As proof of principle, we show functional analysis of somites microinjected with an engineered virus expressing an activated form of Smoothened, the signaling receptor for Sonic Hedgehog (SHH). As adenovirus can infect many embryonic tissues in the mouse, this method may provide an effective alternative to conventional transgenesis for targeted spatial and temporal gene expression. genesis 42:71–76, 2005. © 2005 Wiley‐Liss, Inc.