Premium
Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously
Author(s) -
Shimizukawa Rie,
Sakata Aya,
Hirose Michiko,
Takahashi Akio,
Iseki Hiroyoshi,
Liu Ying,
Kunita Satoshi,
Sugiyama Fumihiro,
Yagami Kenichi
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20122
Subject(s) - chimera (genetics) , embryonic stem cell , green fluorescent protein , biology , induced pluripotent stem cell , blastocyst , microbiology and biotechnology , germline , cell culture , stem cell , transgene , inner cell mass , regenerative medicine , genetics , gene , embryogenesis , embryo
Transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP) are useful as marker lines in chimera experiments. We established a new embryonic stem (ES) cell line (named B6G-2) from a C57BL/6 blastocyst showing ubiquitous EGFP expression. Undifferentiated B6G-2 cells showed strong green fluorescence and mRNAs of pluripotent marker genes. B6G-2 cells were transferred into a C57BL/6 blastocyst to generate a germline chimera, the progeny of which inherited ubiquitous EGFP expression. Mice derived completely from B6G-2 cells were also developed from the ES cells; these were tetraploid chimeras. The established B6G-2 cells were shown to be pluripotent and to be capable of differentiating into cells of all lineages. Thus, the new ES cell line expressing EGFP ubiquitously is useful for basic research in the field of regenerative medicine. The B6G-2 cell line is freely available from the BioResource Center, RIKEN Tsukuba Institute (http://www.brc.riken.jp/lab/cell/english/).