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Keratin 19 gene drives Cre recombinase expression throughout the early postimplantation mouse embryo
Author(s) -
Means Anna L.,
Chytil Anna,
Moses Harold L.,
Coffey Robert J.,
Wright Christopher V.E.,
Taketo Makoto M.,
Grady William M.
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20119
Subject(s) - biology , endoderm , cre recombinase , recombinase , embryo , genetics , cre lox recombination , allele , gene targeting , gene , embryogenesis , microbiology and biotechnology , gene knockin , embryonic stem cell , locus (genetics) , transgene , recombination , genetically modified mouse
The development of Cre‐lox technology has created new opportunities for studying the tissue‐specific functions of genes in vivo during development and disease. We analyzed the spatial and temporal activity of Cre recombinase whose coding sequence was inserted into the endogenous locus for keratin 19. Rather than providing epithelial‐specific recombination during organogenesis, this K19 cre allele allows unexpected recombination in early embryonic development, resulting in recombination of a loxP‐flanked allele throughout all tissues of the mouse, but with sparing of the extraembryonic endoderm, including the anterior visceral endoderm. genesis 42:23–27, 2005. © 2005 Wiley‐Liss, Inc.