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Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse
Author(s) -
Schuler Michael,
Ali Faisal,
Metzger Elisabeth,
Chambon Pierre,
Metzger Daniel
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20107
Subject(s) - cre recombinase , skeletal muscle , biology , somatic cell , transgene , genetically modified mouse , gene , gene targeting , microbiology and biotechnology , human artificial chromosome , genetics , chromosome , anatomy
To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA‐Cre‐ER T2 mouse line in which the expression of the tamoxifen‐dependent Cre‐ER T2 recombinase is under the control of a large genomic DNA segment of the human skeletal muscle α‐actin gene, contained in a P1‐derived artificial chromosome. In this transgenic line Cre‐ER T2 is selectively expressed in skeletal muscles, and Cre‐ER T2 ‐mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle‐specific and strictly tamoxifen‐dependent. HSA‐Cre‐ER T2 mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders. genesis 41:165–170, 2005. © 2005 Wiley‐Liss, Inc.