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Gene expression profile analysis of mouse colon embryonic development
Author(s) -
Park YoungKyu,
Franklin Jeffrey L.,
Settle Stephen H.,
Levy Shawn E.,
Chung Eunkyung,
Jeyakumar Loice H.,
Shyr Yu,
Washington Mary Kay,
Whitehead Robert H.,
Aronow Bruce J.,
Coffey Robert J.
Publication year - 2005
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20088
Subject(s) - biology , mesenchyme , embryogenesis , organogenesis , period (music) , embryonic stem cell , gene expression , endoderm , gene , microarray analysis techniques , microbiology and biotechnology , regulation of gene expression , gene expression profiling , microarray , genetics , embryo , physics , acoustics
During late embryogenesis, the mouse colon develops from a pseudostratified, undifferentiated endoderm to a single‐layered columnar epithelium with accompanying mesenchymal maturation. To identify regulatory genetic programs underlying these morphological changes, we profiled gene expression of the developing mouse colon by microarray from embryonic day (E)13.5 to E18.5. Unbiased cluster analysis of 13,484 cDNA elements revealed two distinct groups of genes whose expression changes reflect the dynamic morphological events of the epithelium and mesenchyme during this period. Additional analyses revealed two subsets of genes whose expression is either upregulated or downregulated over the same developmental period. Of those genes whose expression increases from E13.5 to E18.5 (n = 158), known functions include acquisition and/or maintenance of colonic differentiation. Genes whose transcription is downregulated over this period (n = 49) have demonstrated roles in nuclear organization, transcriptional regulation, and cell proliferation. These results provide the basis for a molecular portrait of colonic development during late embryogenesis and should be a valuable resource for investigators interested in colonic development and neoplasia, as well as comparative organogenesis. genesis 41:1–12, 2005. © 2005 Wiley‐Liss, Inc.