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Characterization of Frzb‐Cre transgenic mouse
Author(s) -
Tylzanowski Przemko,
Bossuyt Wouter,
Thomas J. Terrig,
Luyten Frank P.
Publication year - 2004
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20086
Subject(s) - dkk1 , wnt signaling pathway , transgene , genetically modified mouse , biology , receptor , microbiology and biotechnology , glycoprotein , gene , signal transduction , genetics
Abstract The Wnt family of glycoproteins is involved in numerous developmental and disease processes in higher eukaryotes, exerting their action by binding to cell‐surface receptors. In the extracellular space, Wnts are negatively regulated by secreted antagonists that either bind to the receptors directly (Dkk1) or to Wnt molecules themselves (Sfrp‐FRZB family), preventing its subsequent binding to the receptor. Here we report on a transgenic mouse expressing Cre under the control of the mouse Frzb promoter element. Analysis of the Cre expression was carried out at 10.5 and 14.5 dpc using the ROSA26R mouse line. Expression of the transgenic construct was detected in the limbs, the heart, the nasal epithelium, bone, whiskers, and around the orbita of the eye. The mouse could be used for conditional gene modification in those tissues. genesis 40:200–204, 2004. © 2004 Wiley‐Liss, Inc.