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E1‐Ngn2 /Cre is a new line for regional activation of Cre recombinase in the developing CNS
Author(s) -
Berger Joachim,
Eckert Silke,
Scardigli Raffaella,
Guillemot François,
Gruss Peter,
Stoykova Anastassia
Publication year - 2004
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20081
Subject(s) - cre recombinase , biology , recombinase , enhancer , hindbrain , ganglionic eminence , genetically modified mouse , microbiology and biotechnology , transgene , neuroscience , enhancer trap , progenitor cell , central nervous system , genetics , gene , gene expression , stem cell , recombination
Abstract We generated a transgenic mouse line named E1‐Ngn2/ Cre that expresses Cre recombinase and GFP under the control of the E1 enhancer element of the gene Ngn2 (Scardigli et al.: Neuron 31:203–217, 2001). Cre‐recombinase activity and GFP fluorescence are consistent with the reported expression pattern controlled by the E1‐Ngn2 enhancer. Recombination was detected in the progenitor domains p1 and p2 in the ventricular zone of the neural tube and in distinct domains of the pretectum, the dorsal and ventral thalamus, the tegmentum of the mesencephalon, and the hindbrain. In the developing cortex, Cre‐recombinase activity is confined to a subpopulation of progenitors predominantly in the region of the ventral and lateral pallium. The E1‐Ngn2/ Cre mouse line thus provides an excellent novel tool for a region‐specific conditional mutagenesis in the developing CNS. genesis 40:195–199, 2004. © 2004 Wiley‐Liss, Inc.