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Cre recombinase specificity defined by the tau locus
Author(s) -
KoretsSmith Ella,
Lindemann Lothar,
Tucker Kerry Lee,
Jiang Caoyang,
Kabacs Nikolett,
Belteki Gusztav,
Haigh Jody,
Gertsenstein Marina,
Nagy Andras
Publication year - 2004
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20074
Subject(s) - cre recombinase , biology , transgene , recombinase , locus (genetics) , genetically modified mouse , allele , genetics , gene targeting , cre lox recombination , microbiology and biotechnology , offspring , reporter gene , gene , recombination , gene expression , pregnancy
We generated a transgenic mouse line ( tau ::Cre) by targeting the Cre to the tau locus (Mapt). Based on previous reports on the expression of Tau during development, we expected the Cre recombinase to be expressed in a neuron‐specific and pan‐neuronal manner. However, intercrosses between the tau ::Cre and the Cre‐activatable reporter animals resulted in offspring with recombination either restricted to the nervous system or throughout the entire conceptus, indicating expression of Tau early in development. The percentage of neuron‐specific excision was dependent on the Cre reporter used representing different Cre target sites in the mouse genome. In spite of the observed variability, our data suggest that the tau ::Cre mouse line can be used for pan‐neuronal recombination of floxed alleles when it is used with caution. genesis 40:131–138, 2004. © 2004 Wiley‐Liss, Inc.