Premium
Nuclear β‐catenin‐dependent Wnt8 signaling in vegetal cells of the early sea urchin embryo regulates gastrulation and differentiation of endoderm and mesodermal cell lineages
Author(s) -
Wikramanayake Athula H.,
Peterson Robert,
Chen Jing,
Huang Ling,
Bince Joanna M.,
McClay David R.,
Klein William H.
Publication year - 2004
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20045
Subject(s) - endoderm , biology , microbiology and biotechnology , gastrulation , morpholino , blastula , polarity in embryogenesis , mesoderm , brachyury , mesenchyme , embryo , nodal signaling , embryonic stem cell , embryogenesis , zebrafish , genetics , gene
The entry of β‐catenin into vegetal cell nuclei beginning at the 16‐cell stage is one of the earliest known molecular asymmetries seen along the animal–vegetal axis in the sea urchin embryo. Nuclear β‐catenin activates a vegetal signaling cascade that mediates micromere specification and specification of the endomesoderm in the remaining cells of the vegetal half of the embryo. Only a few potential target genes of nuclear β‐catenin have been functionally analyzed in the sea urchin embryo. Here, we show that SpWnt8 , a Wnt8 homolog from Strongylocentrotus purpuratus, is zygotically activated specifically in 16‐cell‐stage micromeres in a nuclear β‐catenin‐dependent manner, and its expression remains restricted to the micromeres until the 60‐cell stage. At the late 60‐cell stage nuclear β‐catenin‐dependent SpWnt8 expression expands to the veg2 cell tier. SpWnt8 is the only signaling molecule thus far identified with expression localized to the 16–60‐cell stage micromeres and the veg2 tier. Overexpression of SpWnt8 by mRNA microinjection produced embryos with multiple invagination sites and showed that, consistent with its localization, SpWnt8 is a strong inducer of endoderm. Blocking SpWnt8 function using SpWnt8 morpholino antisense oligonucleotides produced embryos that formed micromeres that could transmit the early endomesoderm‐inducing signal, but these cells failed to differentiate as primary mesenchyme cells. SpWnt8‐morpholino embryos also did not form endoderm, or secondary mesenchyme‐derived pigment and muscle cells, indicating a role for SpWnt8 in gastrulation and in the differentiation of endomesodermal lineages. These results establish SpWnt8 as a critical component of the endomesoderm regulatory network in the sea urchin embryo. genesis 39:194–205, 2004. © 2004 Wiley‐Liss, Inc.