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Coat color‐tagged green mouse with EGFP expressed from the RNA polymerase II promoter
Author(s) -
Hsiao YiChun,
Chang HsiaoHui,
Tsai ChingYen,
Jong YiinJeng,
Horng LongShyan,
Lin ShuFen,
Tsai TingFen
Publication year - 2004
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.20038
Subject(s) - biology , transgene , green fluorescent protein , microbiology and biotechnology , gene , complementary dna , gene expression , promoter , coat , genetics , paleontology
Laborious molecular genotyping and variegated gene expression are two widely encountered issues for transgenic mouse studies. To facilitate genotyping in the FVB/N albino background and to reduce variegated expression, we successfully generated double‐tagged transgenic mice for direct visual genotyping with the coat color phenotype derived from tyrosinase cDNA driven by the tyrosinase promoter and with simultaneous high enhanced green fluorescent protein (EGFP) expression driven by the promoter of RNA polymerase II large subunit gene. Incorporation of insulator into a transgene construct achieved high efficiency of transgene expression in more than 90% of the founders. EGFP was detected as early as the one‐cell fertilized egg and lasted for the whole embryo development, as well as in all of the adult tissues examined. The coat color‐tagged green mice offer opportunities in applications such as tissue transplantation, lineage tracing, chimera biology, RNA interference, and other transgenic studies. genesis 39:122–129, 2004. © 2004 Wiley‐Liss, Inc.