Premium
Efficient FLP recombination in mouse ES cells and oocytes
Author(s) -
Schaft Julia,
AsheryPadan Ruth,
van der Hoeven Frank,
Gruss Peter,
Stewart A. Francis
Publication year - 2001
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.1076
Subject(s) - microinjection , recombination , flp frt recombination , recombinase , homologous recombination , microbiology and biotechnology , biology , transfection , site specific recombination , cre lox recombination , genetic recombination , genetics , chemistry , gene , transgene , genetically modified mouse
Summary: We report an improved vector, pCAGGS‐FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS‐FLPe into oocytes, about one‐third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS‐FLPe presents two practical options for removal of FRT cassettes in mice. genesis 31:6–10, 2001. © 2001 Wiley‐Liss, Inc.