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Identification of chromosomal deficiencies that modify Drosophila mastermind mutant phenotypes
Author(s) -
Yedvobnick Barry,
Helms Whitney,
Barrett Britt
Publication year - 2001
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.1071
Subject(s) - phenocopy , biology , notch signaling pathway , phenotype , genetics , hairless , mutant , genetic screen , mutation , gene
Summary: Mastermind (Mam) is a component of Notch pathway signaling. In combination with the intracellular domain of Notch and Suppressor of Hairless, Mam forms a transcriptional activation complex. We have initiated a genetic approach to identify other loci involved in Mam function. The screen utilizes engineered mutations in Mam that derive from GAL4‐UAS‐directed expression of dominant negative constructs. When driven at the wing margin, truncated versions of Mam phenocopy Notch pathway mutations. Correlated with these phenotypes is depression of Notch pathway target expression. Strains expressing truncated versions of Mam were tested for genetic interactions with a large collection of chromosomal deficiencies. Genomic segments that enhanced and suppressed the dominant wing phenotype were identified. These regions may contain uncharacterized loci involved in Notch pathway function. genesis 30:250–258, 2001. © 2001 Wiley‐Liss, Inc.