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Atrial cardiomyocyte‐specific expression of Cre recombinase driven by an Nppa gene fragment
Author(s) -
de Lange Frederik J.,
Moorman Antoon F.M.,
Christoffels Vincent M.
Publication year - 2003
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10220
Subject(s) - cre recombinase , biology , transgene , recombinase , gene , genetically modified mouse , microbiology and biotechnology , transcription (linguistics) , genetics , recombination , linguistics , philosophy
Abstract Summary: To study the development of the atria, we produced a transgenic mouse line that expresses Cre under the regulatory control of a 7 kbp fragment of the Natriuretic peptide precursor type A gene ( Nppa ), from −3 kbp to +4 kbp relative to the transcription start site. Crossing this line with the R26R and Z/EG reporter lines revealed recombinase activity specifically in the cardiomyocytes of the atria and to a lesser extent the inflow tract from E10.5 onwards. At E14.5 recombination in the atria is almost complete. No recombination was observed outside the heart. These mice provide a tool to study gene function in the atria. genesis 37:1–4, 2003. © 2003 Wiley‐Liss, Inc.