Premium
Long‐term, noninvasive imaging of regulated gene expression in living mice
Author(s) -
Hasan Mazahir T.,
Schönig Kai,
Berger Stefan,
Graewe Walter,
Bujard Hermann
Publication year - 2001
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.1014
Subject(s) - luciferase , gene expression , gene , biology , functional genomics , regulation of gene expression , recombinase , cre recombinase , microbiology and biotechnology , computational biology , genomics , genetics , genome , transgene , transfection , genetically modified mouse , recombination
Summary: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long‐term studies of gene function in individual mice. genesis 29:116–122, 2001. © 2001 Wiley‐Liss, Inc.