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Efficient in vitro and in vivo excision of floxed sequences with a high‐capacity adenoviral vector expressing CRE recombinase
Author(s) -
Badorf Michael,
Edenhofer Frank,
Dries Volker,
Kochanek Stefan,
Schiedner Gudrun
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10099
Subject(s) - cre recombinase , recombinase , reporter gene , biology , viral vector , gene , vector (molecular biology) , microbiology and biotechnology , cre lox recombination , transgene , coding region , recombinant dna , in vivo , computational biology , gene expression , genetics , genetically modified mouse , recombination
Summary: Conditional gene expression or gene disruption using Cre/loxP‐ or FLP/frt‐based recombination systems are valuable tools for studying gene function in development and disease. Recombinant adenoviral vectors expressing Cre recombinase have been suggested as an alternative for deletion of floxed sequences. To further improve this approach we generated a high‐capacity adenoviral (HC‐Ad) vector expressing Cre (HC‐Adcre). In this vector all viral coding sequences are deleted resulting in decreased toxicity. In the present study HC‐Adcre efficiently mediated recombination between two loxP sites located in the genome of a reporter cell line. When intravenously injected into ROSA26 reporter mice, a floxed sequence was excised in hepatocytes resulting in expression of the β‐gal reporter. Our data indicate that HC‐Ad vectors expressing Cre effectively delete floxed sequences in vivo and have a significant potential as a tool for functional studies in mice. genesis 33:119–124, 2002. © 2002 Wiley‐Liss, Inc.