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Targeting mammary epithelial cells using a bacterial artificial chromosome
Author(s) -
Wintermantel Tim M.,
Mayer Anja K.,
Schütz Günther,
Greiner Erich F.
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10097
Subject(s) - cre recombinase , bacterial artificial chromosome , recombinase , biology , transgene , genetically modified mouse , microbiology and biotechnology , homologous recombination , gene , mammary gland , lactation , genetics , recombination , genome , breast cancer , pregnancy , cancer
We describe the generation of transgenic mouse lines expressing Cre recombinase in epithelial cells of the lactating mammary gland. As an expression vector, we used a P1‐derived bacterial artificial chromosome (PAC) which harbors the gene for the secretory milk protein, whey acidic protein ( Wap ). Using homologous recombination in E. coli , the PAC was modified to carry the improved coding sequence of Cre recombinase (iCre). Transgenic lines carrying the WAPiCre PAC express Cre recombinase efficiently in the majority of mammary epithelial cells upon lactation. Of only four transgenic lines produced, three express Cre recombinase to a high efficiency. LoxP ‐flanked DNA sequences are recombined in virtually all epithelial cells of WAPiCre transgenic mice at lactation day 3. genesis 33:125–130, 2002. © 2002 Wiley‐Liss, Inc.