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Novel Sxr a ES cell line offers hope for Y chromosome gene‐targeted mice
Author(s) -
Simpson Elizabeth M.,
Johnson Kevin A.,
Shirley BobbiJo,
Fang Ge Y.,
Bayleran Janet K.,
Lerner Charles P.
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10093
Subject(s) - biology , gene , genetics , chromosome , y chromosome , chromosome 21 , chromosome 17 (human) , gene duplication , germ cell , chromosome engineering , embryonic stem cell , germline , microbiology and biotechnology
Summary: A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XY Sxr a mouse, which carries a duplication of the gene‐rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the Y Sxr a chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes. genesis 33:62–66, 2002. © 2002 Wiley‐Liss, Inc.