Premium
DEAF‐1 function is essential for the early embryonic development of drosophila
Author(s) -
Veraksa Alexey,
Kennison James,
McGinnis William
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10090
Subject(s) - biology , hox gene , polytene chromosome , drosophila embryogenesis , primordium , genetics , embryo , gene , microbiology and biotechnology , embryogenesis , zygote , drosophila (subgenus) , function (biology) , embryonic stem cell , drosophila melanogaster , gene expression
Summary: The Drosophila protein DEAF‐1 is a sequence‐specific DNA binding protein that was isolated as a putative cofactor of the Hox protein Deformed (Dfd). In this study, we analyze the effects of loss or gain of DEAF‐1 function on Drosophila development. Maternal/zygotic mutations of DEAF‐1 largely result in early embryonic arrest prior to the expression of zygotic segmentation genes, although a few embryos develop into larvae with segmentation defects of variable severity. Overexpression of DEAF‐1 protein in embryos can induce defects in migration/closure of the dorsal epidermis, and overexpression in adult primordia can strongly disrupt the development of eye or wing. The DEAF‐1 protein associates with many discrete sites on polytene chromosomes, suggesting that DEAF‐1 is a rather general regulator of gene expression. genesis 33:67–76, 2002. © 2002 Wiley‐Liss, Inc.