Premium
Induced DNA recombination by Cre recombinase protein transduction
Author(s) -
Joshi Sunil K.,
Hashimoto Kahoko,
Koni Pandelakis A.
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10089
Subject(s) - cre recombinase , recombinase , cre lox recombination , site specific recombination , transduction (biophysics) , dna , biology , genetics , transgene , recombination , genetic recombination , flp frt recombination , microbiology and biotechnology , genetically modified mouse , gene , biophysics
Summary: Cre is a DNA recombinase that recognizes 34 base‐pair loxP sites of recombination. We have developed a cell‐permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP ‐flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites. genesis 33:48–54, 2002. © 2002 Wiley‐Liss, Inc.