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CDC‐25.1 regulates germline proliferation in Caenorhabditis elegans
Author(s) -
Ashcroft Neville,
Golden Andy
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10083
Subject(s) - germline , caenorhabditis elegans , biology , somatic cell , cell division , genetics , mutant , sterility , germline mutation , embryonic stem cell , mitosis , microbiology and biotechnology , embryo , caenorhabditis , germ cell , mutation , cell , gene
Summary: The cell cycles in C. elegans are tightly controlled but appear to use the same regulators found in other organisms. Four homologues of the dual‐specificity phosphatase Cdc25 are present in the C. elegans genome. In our study, we have characterized a deletion mutant for one of these orthologues. We show that embryonic defects are absent in cdc‐25.1 homozygous mutants, presumably because of maternally contributed CDC‐25.1 product. These embryos hatch and develop into sterile adults. The adults do not appear to have any somatic defects. The sterility results from inadequate germline proliferation. Germline precursors divide slowly and produce abnormally sized daughter cells. Only three to four rounds of germ‐cell division occur before they die during the L3 and L4 larval stages. genesis 33:1–7, 2002. © 2002 Wiley‐Liss, Inc.