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A cre/ lox P‐deleter transgenic line in mouse strain 129S1/SvImJ
Author(s) -
Tang ShihHuey E.,
Silva Francisco J.,
Tsark Walter M.K.,
Mann Jeffrey R.
Publication year - 2002
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10030
Subject(s) - transgene , cre recombinase , biology , genetically modified mouse , embryonic stem cell , recombinase , cre lox recombination , locus (genetics) , microbiology and biotechnology , genetics , gene knockin , gene , recombination
Summary: A Cre recombinase expression cassette was inserted into the X‐linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvImJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this “Cre‐deleter” transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene. genesis 32:199–202, 2002. © 2002 Wiley‐Liss, Inc.