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An enhancer sequence directs LacZ expression to developing pharyngeal endoderm in transgenic mice
Author(s) -
Parmar Hema,
Coletta P. Louise,
Faruque Nadeem,
Sharpe Paul T.
Publication year - 2001
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/gene.10005
Subject(s) - endoderm , foregut , enhancer , biology , homeobox , cdx2 , enhancer trap , gene , microbiology and biotechnology , reporter gene , promoter , transgene , exon , gastrulation , gene expression , genetics , cellular differentiation , embryonic stem cell , anatomy
Summary: The murine Hoxc‐6 homeobox gene comprises three exons with two distinct promoters (PRI and PRII) located 9 kb apart. To characterise the PRII promoter, a region 3 kb upstream of the transcription start site was sequenced, and an Antananapedia‐like consensus binding sequence was found (Coletta et al. , 1991). A LacZ reporter gene construct, containing three copies of this sequence, directs highly specific expression in cells forming pharyngeal endoderm in transgenic mice. Expression was first detected in a few individual anterior endoderm cells at E7.5, which increase in number up to E9.5, where expression was clearly visible in the pharyngeal endoderm. Expression of the endodermal genes HNF3β , Pax‐9 , Shh , and Nkx2.5 showed colocalization with the LacZ ‐positive cells in the foregut and pharyngeal endoderm. This novel enhancer provides a means of tracking the morphogenetic movement of endodermal cells fated to form the foregut. genesis 31:57–63, 2001. © 2001 Wiley‐Liss, Inc.