Open AccessA Highly Reproducible Micro U‐Well Array Plate Facilitating High‐Throughput Tumor Spheroid Culture and Drug Assessment (Global Challenges 2/2021)
Author(s) -
Wu KuangWei,
Kuo ChingTe,
Tu TingYuan
Publication year - 2021
Publication title -
global challenges
Language(s) - English
Resource type - Journals
ISSN - 2056-6646
DOI - 10.1002/gch2.202170021
Subject(s) - spheroid , multicellular organism , cisplatin , drug , throughput , cell culture , cancer research , chemistry , cell , materials science , biology , computer science , chemotherapy , pharmacology , genetics , biochemistry , telecommunications , wireless
In article number 2000056 Ting‐Yuan Tu and co‐workers demonstrate a facile and highly reproducible laser‐ablated micro U‐well with optimal array arrangement. Cell‐loss free formation of multicellular tumor spheroids is performed in 96‐well plates for high‐throughput drug screening of cisplatin. Dose‐response assays are investigated on T24, A549, and Huh‐7 in both 2D and multicellular tumor spheroid (MCTS) conditions. The permeability dynamics of each type of MCTS are examined.