Open AccessHydrogen‐Peroxide‐Generating Electrochemical Scaffold Eradicates Methicillin‐Resistant Staphylococcus aureus Biofilms
Author(s) -
Raval Yash S.,
Mohamed Abdelrhman,
Zmuda Hannah M.,
Patel Robin,
Beyenal Haluk
Publication year - 2019
Publication title -
global challenges
Language(s) - English
Resource type - Journals
ISSN - 2056-6646
DOI - 10.1002/gch2.201800101
Subject(s) - biofilm , staphylococcus aureus , microbiology and biotechnology , antibiotics , methicillin resistant staphylococcus aureus , hydrogen peroxide , bacteria , scaffold , antibiotic resistance , medicine , chemistry , biology , biomedical engineering , genetics , organic chemistry
Increasing rates of chronic wound infections caused by antibiotic‐resistant bacteria are a crisis in healthcare settings. Biofilms formed by bacterial communities in these wounds create a complex environment, enabling bacteria to persist, even with antibiotic treatment. Wound infections caused by methicillin‐resistant Staphylococcus aureus (MRSA) are major causes of morbidity in clinical practice. There is a need for new therapeutic interventions not based on antibiotics. Hydrogen peroxide (H 2 O 2 ) is a known antibacterial/antibiofilm agent, continuous delivery of which has been challenging. A conductive electrochemical scaffold (e‐scaffold) is developed, which is composed of carbon fabric that electrochemically reduces dissolved oxygen into H 2 O 2 when polarized at −0.6 V Ag/AgCl , as a novel antibiofilm wound dressing material. In this study, the in vitro antibiofilm activity of the e‐scaffold against MRSA is investigated. The developed e‐scaffold efficiently eradicates MRSA biofilms, based on bacterial quantitation and ATP measurements. Moreover, imaging hinted at the possibility of cell‐membrane damage as a mechanism of action. These results suggest that an H 2 O 2 ‐generating e‐scaffold may be a novel platform for eliminating MRSA biofilms without using antibiotics and may be useful to treat chronic MRSA wound infections.