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Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two‐color fluorescence in situ hybridization (FISH)
Author(s) -
Smolarek Teresa A.,
Blough Ruthann I.,
Foster Richard S.,
Ulbright Thomas M.,
Palmer Catherine G.,
Heerema Nyla A.
Publication year - 1995
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.2870140403
Subject(s) - fluorescence in situ hybridization , biology , microdissection , chromosome 12 , polymerase chain reaction , chromosome , in situ hybridization , microbiology and biotechnology , pathology , germ cell tumors , genetics , gene , chemotherapy , medicine , gene expression
The distribution of segments of the short and long arms of chromosome 12 was distinguished by two‐color fluorescence in situ hybridization (FISH) in 27 cytogenetically abnormal testicular germ cell tumors (TGCTs). A 12p‐specific probe was developed by chromosomal microdissection and sequence‐independent polymerase chain reaction (PCR) amplification and was combined with a commercially available whole‐chromosome 12 painting probe. The TGCTs included both i(12p)‐positive and i(12p)‐negative primary tumors and lymph node metastases from patients in clinical stage I or stage II who were not previously treated with chemotherapy. Rearrangements of the short arm of chromosome 12 and overrepresentation of 12p DNA sequences were found in all cases. In addition, cryptic rearrangements of 12p were found in 39% (7/18) of the i(12p)‐positive tumors and in 78% (7/9) of the i(12p)‐negative tumors. Only 7% (2/27) of all tumors had cryptic rearrangements of 12q.